Poult. Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McDaniel, C.
Right arrow Articles by Zumwalt, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McDaniel, C.
Right arrow Articles by Zumwalt, C.
Poultry Science, Vol 77, Issue 6, 888-893
Copyright © 1998 by Poultry Science Association

Articles

Use of a sperm analyzer for evaluating broiler breeder males. 1. Effects of altering sperm quality and quantity on the sperm motility index

CD McDaniel, JL Hannah, HM Parker, TW Smith, CD Schultz, and CD Zumwalt

Poultry Science Department, Mississippi State University, Mississippi State 39762, USA. cmcdaniel@poultry.msstate.edu

A new instrument for assessing mammalian semen attributes, the Sperm Quality Analyzer, was evaluated as a potential tool for determining rooster sperm quality. The Sperm Quality Analyzer measures the "activity" of sperm in a semen sample as the sperm motility index (SMI). The SMI is defined as the number and amplitude of deflections in a light path per second as a result of sperm movement within a capillary tube. In the present study, effects of sperm concentration, viability, and motility on the SMI were evaluated. Peterson broiler breeder males (n = 40) were used as semen donors. In the initial experiment, semen was diluted from 2- to 25-fold and SMI readings were obtained. The SMI was very low in neat semen samples but increased when semen was diluted up to threefold. However, at dilutions greater than fivefold, the SMI decreased. Apparently, sperm concentration in undiluted semen is so great that sperm are unable to move freely within the capillary tube. Maximum SMI values were obtained at sperm concentrations of approximately 1 billion sperm per milliliter. When thawed, dead sperm were mixed with incubated, live sperm, the SMI decreased with decreasing sperm viability even though sperm concentration was constant. Obviously, fewer sperm move across the light beam as sperm mortality increases. When motile, aerobically incubated sperm were mixed at different rates with immotile, anaerobically incubated sperm samples, the SMI increased with increasing concentrations of motile sperm, whereas total sperm concentration was static. In addition, the SMI was strongly correlated with motility scores obtained by microscopic analysis. The Sperm Quality Analyzer provides an estimate of the overall quality of sperm from broiler breeder males by reflecting sperm concentration, viability, and motility in a single value, the SMI.


This article has been cited by other articles:


Home page
 Poult. Sci.Home page
S. J. Liu, J. X. Zheng, and N. Yang
Semen Quality Factor as an Indicator of Fertilizing Ability for Geese
Poult. Sci., January 1, 2008; 87(1): 155 - 159.
[Abstract] [Full Text] [PDF]

Home page
 Poult. Sci.Home page
P. R. Dumpala, H. M. Parker, and C. D. McDaniel
Similarities and Differences Between the Sperm Quality Index and Sperm Mobility Index of Broiler Breeder Semen
Poult. Sci., December 1, 2006; 85(12): 2231 - 2240.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Poultry Science Association.