Induction of the delayed footpad and wattle reaction to killed Staphylococcus aureus in chickens

Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.

Two experiments were conducted to induce the delayed footpad reaction (DFR) to killed Staphylococcus aureus antigen. In Experiment 1, tracheal, cloacal, and choanal swabs were collected from chickens prior to sensitization with S. aureus to determine the carrier status of S. aureus. The second experiment compared the DFR to the delayed wattle reaction (DWR). Chickens were subjected to single or multiple sensitizations in the neck with S. aureus antigen between 4 and 6 wk of age. One week later, birds were challenged with S. aureus either in the right footpad or wattle. The left footpad or wattle was injected with PBS. The thicknesses of the footpad or the wattle were measured up to 96 h postchallenge. The recoveries of S. aureus from the choanal slit and trachea were significantly higher than that of the cloaca (P < 0.001). Birds of Experiment 1 showed a significant DFR (P < 0.0001) following intradermal challenge with killed S. aureus that was sustained through 48 h postchallenge with no difference in the DFR between carrier and noncarrier birds. In Experiment 2, the thicknesses of the footpad and wattle were significantly increased following challenge with S. aureus (P < 0.0001), with the footpad showing a greater response than the wattle (P < 0.001). Three sensitizing dosages, as compared to two dosages, resulted in a less pronounced DFR and DWR (P < 0.02). These results indicate that the DFR can be used as a delayed reaction model in the study of staphylococcosis in poultry.