Poult. Sci.
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Poultry Science, Vol 82, Issue 11, 1726-1732
Copyright © 2003 by Poultry Science Association

Articles

Efficacy of a phytase derived from Escherichia coli and expressed in yeast on phosphorus utilization and bone mineralization in turkey poults

TJ Applegate, DM Webel, and XG Lei

Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA. applegt@purdue.edu

A slope-response bioassay was conducted with male turkey poults to determine the sparing effect of P, based on improvements in bone mineralization in turkey poults, from 10 to 21 d of age when diets were supplemented with a novel phytase. Reference diets for calculation of the sparing effect of P contained 0.47, 0.55, 0.70, and 0.79% nonphytate phosphorus (NPP). Diets with varying dosages of a swine, Escherichia coli-derived AppA2 phytase (ECP) expressed in Pichia pastoris yeast (0, 250, 500, 750, and 1,000 U/kg) were added to the 0.47% NPP diet and improvements in bone mineralization determined the sparing effect of P supplied from ECP. Two additional reference diets were included that contained 500 U/kg from one of two commercial phytases (PA and PB) derived from Aspergillus and Peniophora. At 500 U/kg diet the ECP spared an additional 0.22% NPP (if calculated from tibia ash %), 0.18% NPP (if calculated from toe ash %), 0.24% NPP (if calculated from mg tibia ash), or 0.21% NPP (if calculated from mg toe ash). Phosphorus retention results validate bioassay results, in that 500 U ECP/kg resulted in 68.2% P being retained (0.49% of diet P retained) as compared with only 58.9% P being retained from the unsupplemented control diet (0.421% of diet P retained; P < 0.05).


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