Poult. Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leshchinsky, T.
Right arrow Articles by Klasing, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leshchinsky, T.
Right arrow Articles by Klasing, K.
Poultry Science, Vol 82, Issue 8, 1266-1273
Copyright © 2003 by Poultry Science Association

Articles

Profile of chicken cytokines induced by lipopolysaccharide is modulated by dietary alpha-tocopheryl acetate

TV Leshchinsky and KC Klasing

Department of Animal Science, University of California, Davis, California 95616, USA.

Previously, we found that 25 to 50 IU/kg of dietary vitamin E (VE) had very different immunoregulatory effects than high VE levels (200 IU/kg), and we hypothesized that this difference was due to different cytokine profiles. Chicks were fed 0, 30, or 200 IU/kg supplemental VE and percentages of CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- lymphocytes, and the ratio of CD4+/CD8+ lymphocytes was determined. The expression of chicken splenic interleukin-1beta (IL-1beta), myelomonocytic growth factor (MGF), interferon (IFN-gamma), and transforming growth factor-beta (TGFbeta) mRNA was determined by reverse transcription (RT)-PCR after intravenous injection of lipopolysaccharide (LPS). Due to a tendency for increased CD4-CD8+ lymphocytes at 30 IU/kg VE (P=0.072), the CD4+/CD8+ ratio was significantly lower for 30 IU/kg VE compared with 0 IU/kg VE (P=0.041). The VE dose of 200 IU/kg decreased the constitutive (prior to LPS) expression of TGFbeta. The LPS caused an increase in IL-1beta, MGF, and IFNgamma expression at all VE concentrations and had no effect on IL-2 and TGFbeta mRNA expression. Dietary VE decreased MGF mRNA (P=0.049) in a dose-dependent manner but had no effect on the expression of other cytokines. The decreased expression of MGF could explain the immunomodulatory effect of VE in inflammation.


This article has been cited by other articles:


Home page
 Poult. Sci.Home page
A. M. A. Mageed, N. Isobe, and Y. Yoshimura
Expression of Avian {beta}-Defensins in the Oviduct and Effects of Lipopolysaccharide on Their Expression in the Vagina of Hens
Poult. Sci., May 1, 2008; 87(5): 979 - 984.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. Subedi, N. Isobe, M. Nishibori, and Y. Yoshimura
Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus)
Reproduction, January 1, 2007; 133(1): 127 - 133.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Poultry Science Association.