HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Wang, Q. Articles by Wang, Y. Search for Related Content PubMed PubMed Citation Articles by Wang, Q. Articles by Wang, Y.

Tissue Expression and Association with Fatness Traits of Liver Fatty Acid-Binding Protein Gene in Chicken¹

Q. Wang^*, H. Li^*,2, N. Li, L. Leng^* and Y. Wang^*

^* College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; and National Laboratories for Agribiotechnology, China Agricultural University, Beijing 100094, China

² Corresponding author: lihui{at}neau.edu.cn

Fatty acid-binding proteins belong to a superfamily of lipid-binding proteins that exhibit a high affinity for long-chain fatty acids and appear to function in metabolism and intracellular transportation of lipids. The current study was designed to investigate expression characterization and association with growth and composition traits of the liver fatty acid-binding protein (L-FABP) gene in the chicken. Northern blot analysis indicated that the gene, similar to the mammal L-FABP gene, was expressed only in liver and intestinal tissues. The mRNA levels of the chicken L-FABP gene in liver and intestine had significant differences between the broilers and Baier layers. The China Agricultural University F₂ population was used in the present study. Body weight and body composition traits were measured in the populations. Primers for the coding region and 5' upstream region of the L-FABP gene were designed according to chicken genomic and cDNA sequence. Polymorphisms were detected by DNA sequencing, and the PCR single-strand conformation polymorphisms method was developed to genotype the F₂ population. The results indicated that the L-FABP gene polymorphisms were associated with abdominal fat weight and percentage of abdominal fat, and the L-FABP gene could be a candidate locus or linked to a major gene(s) that affects fatness traits in the chicken. The results of the current study provided basic molecular information for studying the role of the L-FABP gene in the regulation of lipid metabolism in avian species.

Key Words: liver fatty acid-binding protein gene • tissue expression • polymorphism • fatness trait • chicken

¹ This research was supported by the National Natural Science Foundation Key Project (no.30430510) and Program for New Century Excellent Talents in University (no. NCET-04-0343).

This article has been cited by other articles:

				D. Crump, S. Chiu, C. Egloff, and S. W. Kennedy Effects of Hexabromocyclododecane and Polybrominated Diphenyl Ethers on mRNA Expression in Chicken (Gallus domesticus) Hepatocytes Toxicol. Sci., December 1, 2008; 106(2): 479 - 487. [Abstract] [Full Text] [PDF]