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Molecular Cloning, Genomic Organization, and Expression of Three Chicken 5'-AMP-Activated Protein Kinase Gamma Subunit Genes¹

Growth Biology Laboratory, Animal and Natural Resources Institute, USDA-ARS, Beltsville, MD 20705

² Corresponding author: monika{at}anri.barc.usda.gov

The 5'-AMP-activated protein kinase (AMPK) plays a key role in regulating cellular energy homeostasis. The AMPK is a heterotrimeric enzyme complex that consists of 1 catalytic () and 2 regulatory (ß and ) subunits. Mutations of the subunit genes are known to affect AMPK functioning. In this study, we characterized the genomic organization and expression of 3 chicken AMPK subunit genes (cPRKAG). Alternative splicing of the second exon of the cPRKAG1 gene resulted in 2 transcript variants that code for predicted proteins of 298 and 276 amino acids. Use of an alternate promoter and alternative splicing of the cPRKAG2 gene resulted in 4 transcript variants that code for predicted proteins of 567, 452, 328, and 158 amino acids. Alternative splicing of exon 3 of the cPRKAG3 gene resulted in the production of "long" and "short" transcript variants that code for predicted proteins of 382 and 378 amino acids, respectively. We found evidence for differential expression of individual subunit gene transcript variants and, in some cases, tissue-specific expression was observed. The cPRKAG subunit genes displayed similar structural features and high sequence homology compared with corresponding mammalian subunit gene homologues.

Key Words: 5'-AMP-activated protein kinase • gamma subunit • alternative splicing • alternate promoter usage • energy homeostasis

¹ Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other suitable products.

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