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Differential Expression of Mitochondrial and Extramitochondrial Proteins in Lymphocytes of Male Broilers with Low and High Feed Efficiency¹

K. Lassiter^*, C. Ojano-Dirain^*, M. Iqbal^*, N. R. Pumford^*, N. Tinsley^*, J. Lay, R. Liyanage, T. Wing, M. Cooper and W. Bottje^*,2

^* Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville 72701; State-Wide Mass Spectrometry Lab, University of Arkansas, Fayetteville 72701; and Cobb-Vantress, Inc., Siloam Springs, AR 72761

² Corresponding author: wbottje{at}uark.edu

Studies were conducted to investigate relationships between mitochondrial and extramitochondrial protein expression, and protein oxidation in lymphocytes obtained from broilers in which individual feed efficiencies were obtained. Lymphocytes were isolated from male broilers from a single line that were shown to exhibit either low (0.48 ± 0.02, n = 8) or high (0.68 ± 0.01, n = 7) feed efficiency (FE). Western blot analysis showed that, compared with lymphocytes from high FE broilers, lymphocytes from low FE broilers exhibited a) higher amounts of oxidized proteins (protein carbonyls), b) lower amounts of 3 mitochondrial proteins [core I, cyt c 1 (complex III), and ATP synthase (complex V)], and c) higher amounts of 2 proteins [30 S (complex II) and COX II (complex IV)]. Two-dimensional gel electrophoresis revealed that the intensities of 25 protein spots from pooled samples of lymphocytes from high and low FE broilers differed by 5-fold or more. Three of these protein spots were picked from the gel and subjected to matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. One protein spot of ~33 kDa was tentatively identified by MALDI-TOF as a fragment of collapsin-2, a component of semaphorin 3D. The results of this study provide further evidence of increased oxidation associated with low FE and further evidence of differential protein expression associated with the phenotypic expression of feed efficiency.

Key Words: feed efficiency • lymphocytes • mitochondria • protein expression

¹ This research was funded by USDA-NRI grant (#2001-03443), and Cobb-Vantress, Inc. to W. Bottje and M. Iqbal, and is published with support by the Director of the Agriculture Research Experiment Station, University of Arkansas, Fayetteville.

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