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The Expression of Pituitary Gland Genes in Laying Geese¹

C. F. Yen^*, H. W. Lin^*, J. C. Hsu, C. Lin^*, T. F. Shen^* and S. T. Ding^*,2

^* Department of Animal Science and Technology, National Taiwan University, Taipei 106, Taiwan; and Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan

² Corresponding author: sding{at}ccms.ntu.edu.tw

The purpose of this study was to detect differential expression of genes in the pituitary gland in laying geese by suppression subtractive hybridization (SSH). Pituitary glands from prelaying and laying geese were dissected for mRNA extraction. The cDNA from pituitary glands of prelaying geese was subtracted from the cDNA from the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. We screened 384 clones with possible differentially expressed gene fragments by differential screening. Sixty-five clones from the differential screening results were subjected to gene sequencing and further analysis. We found that at least 19 genes were highly expressed in the pituitary glands of laying geese compared with prelaying geese. Among these, 6 genes (including 4 novel genes) were confirmed by virtual Northern analysis. We found that prolactin and visinin-like protein were highly expressed in the pituitary glands of laying geese compared with prelaying geese (P < 0.05). Further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.

Key Words: laying geese • pituitary gland • prolactin

¹ This work was supported by the Council of Agriculture in Taiwan.

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				S. T. Ding, C. F. Yen, P. H. Wang, H. W. Lin, J. C. Hsu, and T. F. Shen The Differential Expression of Hepatic Genes Between Prelaying and Laying Geese Poult. Sci., June 1, 2007; 86(6): 1206 - 1212. [Abstract] [Full Text] [PDF]