HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Froman, D. P. Articles by Rhoads, D. D. Search for Related Content PubMed PubMed Citation Articles by Froman, D. P. Articles by Rhoads, D. D.

An Expressed Sequence Tag Analysis of the Chicken Reproductive Tract Transcriptome

D. P. Froman^*,1, J. D. Kirby and D. D. Rhoads^,

^* Department of Animal Sciences, Oregon State University, Corvallis 97330; and Departments of Poultry Science and Biological Science, University of Arkansas, Fayetteville 72701

¹ Corresponding author: drhoads{at}uark.edu

Analysis of the chicken reproductive tract transcriptome is important in comparative biology for analysis of reproductive tract development and evolution. In addition, molecular analysis of the reproductive tract is important for identification of genes affecting fertility in the poultry industry. We sampled the chicken reproductive tract (ovary, oviduct, and testis) transcriptome, generating 5,328 expressed sequence tags that assembled into 4,518 contigs. We identified 475 contigs with no match in the current expressed sequence tag databases or in GenBank. The novel contigs included 31 with no match to the current assembly of the chicken genome, 119 representing spliced transcripts, and 309 that were unspliced. More detailed molecular characterization of the 428 novel contigs present in the assembly will be important to gene discovery and annotation of the chicken and other vertebrate genomes.

Key Words: expressed sequence tag • chicken • reproductive tract • testis • ovary