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Characterization of the Chicken Small Intestine Type IIb Sodium Phosphate Cotransporter¹

F. Yan^*,2, R. Angel^* and C. M. Ashwell^,3,4

^* Department of Animal and Avian Sciences, University of Maryland, College Park 20742; and Growth Biology Laboratory, Animal and Natural Resources Institute, USDA-ARS, Beltsville, MD 20705

⁴ Corresponding author: chris_ashwell{at}ncsu.edu

Intestinal absorption and renal resorption play a critical role in overall phosphorus homeostasis in chickens. Using RNase-ligase-mediated rapid amplification of cDNA ends PCR, we obtained a cDNA from the broiler small intestine that encodes a type IIb Na-dependent phosphate transporter. The cDNA has an open reading frame of 2,022 bp and predicts a 674-amino acid protein with a molecular mass of approximately 74 kDa. Prediction of membrane spanning domains based on the hydrophilic and hydrophobic properties of the amino acids suggests 8 transmembrane domains, with both the NH₂ and COOH termini being intracellular. The Na-inorganic phosphate (Pi) IIb cotransporter has relative high homology with other type II Na-Pi cotransporters but low homology with the type I or type III Na-Pi cotransporters. Northern blot analysis demonstrated the presence of a single mRNA transcript present predominantly in the small intestine, with the highest expression in the duodenum, followed by the jejunum and ileum. In situ hybridization indicated that the Na-Pi cotransporter mRNA is expressed throughout the vertical cryptvillus axis of the small intestine. Reduction of P in the diet of chicks from hatch to 4 d of age resulted in a significant induction of Na-Pi cotransporter mRNA expression in the small intestine. Further study is needed to elucidate its physiological role in intestinal phosphate absorption in chickens.

Key Words: phosphate • transporter • cloning • small intestine • chicken

¹ The nucleotide sequence data reported in this paper have been submitted to GenBank, AY389468 [GenBank] .

² Current address: Department of Poultry Science, University of Arkansas, Fayetteville 72701.

³ Current address: Department of Poultry Science, North Carolina State University, Raleigh 27695.

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