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Migration and Proliferation of Primordial Germ Cells in the Early Chicken Embryo

Y. Nakamura^*,#, Y. Yamamoto, F. Usui, T. Mushika^*, T. Ono^*, A. R. Setioko, K. Takeda, K. Nirasawa^#, H. Kagami^* and T. Tagami^#,1

^* Department of Food Production Science, Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano 399-4598, Japan; Department of Bioscience and Food Production, Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minamiminowa, Nagano 399-4598, Japan; Department of Poultry and Minor Livestock Program, Indonesian Research Institute for Animal Production (IRIAP), PO Box 221, Bogor 16002, Indonesia; Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Science (NILGS), Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan; and ^# Animal Breeding and Reproduction Research Team, National Institute of Livestock and Grassland Science (NILGS), Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan

¹ Corresponding author: tagami{at}affrc.go.jp

In avian species, primordial germ cells (PGC) use the vascular system as a vehicle to transport them to the future gonadal region. The aim of this study was to elucidate the details of migration system and size of the PGC population in the early chicken embryo. We analyzed whole chicken embryos during stages X and 2 to 17 by immunohistochemical staining using specific antibody raised against chicken vasa homolog. At stage X, PGC were dense in the central zone of the area pellucida. Following the formation of the primitive streak, PGC moved anteriorly to the edge of the extraembryonic region. The size of the PGC population increased gradually during stages X (130.4 ± 31.9) to 10 (439.3 ± 93.6). At stage 10, PGC began to accumulate in the region anterior to the head, and then we could observe that PGC invaded into the vascular system in this region. At stage 11, the number of PGC decreased in the region anterior to the head (129.8 ± 42.5 to 46.7 ± 4.2) and increased in the blood vessels (194.0 ± 41.6 to 285.0 ± 7.5). No PGC could be recognized in the intermediate mesoderm, the future gonadal region, until stage 14, but they first appeared there at stage 15. The number of PGC recognized in the intermediate mesoderm increased from stage 15 to 17. Interestingly, the number of PGC between the left and right sides of this region was consistently and significantly different (P < 0.05) in females and males. The present study mainly clarified that chicken PGC continue to proliferate throughout early development, many PGC invaded into the vascular system from the region anterior to the head in stage 11, and PGC actively left the blood vessels and migrated to the intermediate mesoderm from stage 15.

Key Words: primordial germ cell • vasa • early embryo • immunostaining • chicken

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