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An Assay for Measuring the Mannan-Binding Lectin Pathway of Complement Activation in Chickens

Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, P.O. Box 50, DK-8830 Tjele, Denmark

¹ Corresponding author: liselotter.norup{at}agrsci.dk

To investigate the ability of chicken mannan-binding lectin (cMBL) to work as a complement activator, a heterogeneous ELISA test was developed, in which the deposition of human complement factor 4 (C4) was used as a measure of complement activation ability. Serum from different experimental chicken lines was tested. The correlation between serum cMBL concentrations and human C4 deposition was high (correlation = 0.8549, P < 0.0001). There was no difference in C4 deposition among sera from the chicken lines when calculated as C4 deposition relative to the cMBL concentration.

Key Words: chicken • mannan-binding lectin • mannan-binding lectin-associated serine protease 2 • complement activation • complement factor 4 deposition