HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhou, H. Articles by Sharif, S. Search for Related Content PubMed PubMed Citation Articles by Zhou, H. Articles by Sharif, S.

Appropriate Chicken Sample Size for Identifying the Composition of Broiler Intestinal Microbiota Affected by Dietary Antibiotics, Using the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technique

H. Zhou^*,, J. Gong^*,1, J. T. Brisbin, H. Yu^*, B. Sanei, P. Sabour^* and S. Sharif^,1

^* Food Research Program, Agriculture and Agri-Food Canada, Guelph, Ontario, N1G 5C9, Canada; Department of Poultry Science, Texas A&M University, College Station 77845; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada; and Ontario Ministry of Agriculture, Food and Rural Affairs, Guelph, Ontario N1G 2W1, Canada

¹ Corresponding authors: GongJ{at}agr.gc.ca; shayan{at}uoguelph.ca

The bacterial microbiota in the broiler gastrointestinal tract are crucial for chicken health and growth. Their composition can vary among individual birds. To evaluate the composition of chicken microbiota in response to environmental disruption accurately, 4 different pools made up of 2, 5, 10, and 15 individuals were used to determine how many individuals in each pool were required to assess the degree of variation when using the PCR-denaturing gradient gel electrophoresis (DGGE) profiling technique. The correlation coefficients among 3 replicates within each pool group indicated that the optimal sample size for comparing PCR-DGGE bacterial profiles and downstream applications (such as identifying treatment effects) was 5 birds per pool for cecal microbiota. Subsequently, digesta from 5 birds was pooled to investigate the effects on the microbiota composition of the 2 most commonly used dietary antibiotics (virginiamycin and bacitracin methylene disalicylate) at 2 different doses by using PCR-DGGE, DNA sequencing, and quantitative PCR techniques. Thirteen DGGE DNA bands were identified, representing bacterial groups that had been affected by the antibiotics. Nine of them were validated. The effect of dietary antibiotics on the microbiota composition appeared to be dose and age dependent. These findings provide a working model for elucidating the mechanisms of antibiotic effects on the chicken intestinal microbiota and for developing alternatives to dietary antibiotics.

Key Words: chicken • intestinal microbiota • sample size • dietary antibiotic

This article has been cited by other articles:

				J. T. Brisbin, J. Gong, C. A. Lusty, P. Sabour, B. Sanei, Y. Han, P. E. Shewen, and S. Sharif Influence of In-Feed Virginiamycin on the Systemic and Mucosal Antibody Response of Chickens Poult. Sci., October 1, 2008; 87(10): 1995 - 1999. [Abstract] [Full Text] [PDF]