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Gene Expression in Breast Muscle and Duodenum from Low and High Feed Efficient Broilers¹

C. Ojano-Dirain^*,2, M. Toyomizu, T. Wing, M. Cooper and W. G. Bottje^*

^* Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville 72701; Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-855, Japan; and Cobb-Vantress Inc., Siloam Springs, AR 72761-1030

² Corresponding author: cojano{at}uark.edu

This study was conducted to evaluate messenger RNA (mRNA) expression of genes that are involved in energy metabolism and mitochondrial biogenesis: avian adenine nucleotide translocator (avANT), cytochrome oxidase III (COX III), inducible nitric oxide synthase (iNOS), peroxisome proliferator-activated receptor- (PPAR-), avian PPAR- coactivator-1 (avPGC-1), and avian uncoupling protein in breast muscle and duodenum of broilers with low and high feed efficiency (FE). Total RNA was extracted from snap-frozen tissues from male broilers with low (0.55 ± 0.01) and high (0.72 ± 0.01) FE (n = 8 per group). Total RNA was reverse-transcribed using oligo(dT), random primers, or both followed by real-time reverse transcription-PCR. Protein oxidation, measured as protein carbonyls, was also evaluated in duodenal mucosa. Protein carbonyls were higher in low FE mucosa in tissue homogenate and mitochondrial fraction. The mRNA expression of iNOS and PPAR- in the duodenum was lower in the low FE broilers, with no differences in avANT, COX III, and avPGC-1. In contrast, expression of avANT and COX III mRNA in breast muscle was lower in low FE broilers with no differences in iNOS, PPAR-, and avPGC-1. The avian uncoupling protein in breast muscle was higher in low FE birds (P = 0.068). These results indicate that there are differences in the expression of mRNA encoding for mitochondrial transcription factors and proteins in breast muscle and duodenal tissue between low and high FE birds. The differences that were observed may also reflect inherent metabolic and gene regulation differences between tissues.

Key Words: feed efficiency • broiler • breast muscle • duodenum • gene expression • carbonyl

¹ This research was funded by in part by Cobb-Vantress Inc. and is published with the support of the director of the Agricultural Reseach Station, University of Arkansas, Fayetteville, AR 72701.

This article has been cited by other articles:

				W. G. Bottje and G. E. Carstens Association of mitochondrial function and feed efficiency in poultry and livestock species J Anim Sci, April 1, 2009; 87(14_suppl): E48 - E63. [Abstract] [Full Text] [PDF]