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The Effect of a Commercial Enzyme Preparation on Apparent Metabolizable Energy, the True Ileal Amino Acid Digestibility, and Endogenous Ileal Lysine Losses in Broiler Chickens

S. M. Rutherfurd^*, T. K. Chung and P. J. Moughan

^* Institute of Food, Nutrition and Human Health; Riddet Centre, Massey University, Palmerston North, New Zealand; and DSM Nutritional Products Asia Pacific Pte Ltd., Singapore

¹ Corresponding author: S.M.Rutherfurd{at}massey.ac.nz

The effect of a commercial enzyme preparation containing xylanase, -amylase, and ß-glucanase on dietary AME content and the apparent and true ileal amino acid digestibility of a corn-soy broiler diet and endogenous ileal lysine flow was determined. Two predominantly corn-soy diets also containing wheat bran and canola meal were formulated; one diet contained no added enzymes, whereas the other was supplemented with -amylase, ß-glucanase, and xylanase. Titanium dioxide was included as an indigestible marker. The diets were given to broiler chickens, and AME and true ileal amino acid digestibility were determined. Portions of the 2 test diets were guanidinated and fed to similar aged broiler chickens and endogenous lysine flows determined. The chickens appeared healthy throughout the study, and the mean bird weights at the time of slaughter were not significantly different (P < 0.05) among any of the treatment groups. Dietary AME content was significantly (P < 0.05) higher for the enzyme-supplemented corn-soy diet (2,829 kcal/kg) compared with its unsupplemented control diet (2,766 kcal/kg). True ileal amino acid digestibility was significantly (P < 0.05) higher for all amino acids investigated. The increase ranged from 4% for arginine and glutamic acid to 12% for cystine. There was no significant difference in endogenous ileal lysine flow between broilers fed the unsupplemented diet and those fed the enzyme-supplemented diet. Overall, enzyme supplementation with an enzyme blend containing -amylase, ß-glucanase, and xylanase increased the AME content of a corn-soy broiler diet as well as apparent and true ileal amino acid digestibility for all amino acids, but had no effect on endogenous ileal lysine flow.

Key Words: lysine • digestibility • broiler • endogenous • corn-soy

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