HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ploegaert, T. C. W. Articles by Parmentier, H. K. Search for Related Content PubMed PubMed Citation Articles by Ploegaert, T. C. W. Articles by Parmentier, H. K.

Intratracheally Administered Pathogen-Associated Molecular Patterns Affect Antibody Responses of Poultry

T. C. W. Ploegaert^*,, G. De Vries Reilingh^*, M. G. B. Nieuwland^*, A. Lammers^*, H. F. J. Savelkoul and H. K. Parmentier^*,1

^* Section of Immunology, Adaptation Physiology Group, and Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, the Netherlands

¹ Corresponding author: Henk.Parmentier{at}wur.nl

Various potential immune-modulating microbially derived pathogen-associated molecular patterns (PAMP), or so called homotopes, are present in high concentrations in the environment of food animals. In previous studies, intravenously administered PAMP had variable effects on specific primary and secondary immune responses of poultry to systemically administered antigens. In the present study, we evaluated the effects of intratracheal (i.t.) challenge with the PAMP lipopolysaccharide, lipoteichoic acid (LTA), and Zymosan-A (containing 1,3 ß-glucan) on primary and secondary (total) antibody (Ab) responses and (isotype) IgM, IgG, and IgA responses to systemically administered human serum albumin (HuSA), and Ab titers to infectious bursal disease (Gumboro virus) and infectious bronchitis vaccines in layer hens at 9 and 22 wk of age. Birds were challenged via the trachea with PAMP for 5 consecutive days prior to primary and secondary immunization with HuSA. Intratracheally administered LTA and, to a minor extent, lipopolysaccharide significantly enhanced secondary total and IgG Ab responses to HuSA. 1,3 ß-Glucan did not significantly affect Ab responses to HuSA. All birds challenged with PAMP showed a decreased BW. Higher total Ab titers to infectious bursal disease and infectious bronchitis were found in birds challenged with LTA. The present results indicate that i.t. administered PAMP affect the humoral immune responsiveness of poultry, which may lead to an enhanced status of immune reactivity. Furthermore, our results suggest that the hygienic status of the environment influences BW (gain). The consequences of immune modulation by airborne PAMP or hygienic conditions in chicken husbandry for vaccine delivery and immune responsiveness of poultry are discussed.

Key Words: pathogen-associated molecular pattern • modulation • vaccine • intratracheal • airborne

This article has been cited by other articles:

				L. Star, H. R. Juul-Madsen, E. Decuypere, M. G. B. Nieuwland, G. de Vries Reilingh, H. van den Brand, B. Kemp, and H. K. Parmentier Effect of early life thermal conditioning and immune challenge on thermotolerance and humoral immune competence in adult laying hens Poult. Sci., November 1, 2009; 88(11): 2253 - 2261. [Abstract] [Full Text] [PDF]

				H. T. L. Lai, M. G. B. Nieuwland, B. Kemp, A. J. A. Aarnink, and H. K. Parmentier Effects of dust and airborne dust components on antibody responses, body weight gain, and heart morphology of broilers Poult. Sci., September 1, 2009; 88(9): 1838 - 1849. [Abstract] [Full Text] [PDF]

				H. K. Parmentier, T. B. Rodenburg, G. De Vries Reilingh, B. Beerda, and B. Kemp Does enhancement of specific immune responses predispose laying hens for feather pecking? Poult. Sci., March 1, 2009; 88(3): 536 - 542. [Abstract] [Full Text] [PDF]