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MOLECULAR, CELLULAR, AND DEVELOPMENTAL BIOLOGY |
* Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061; and Aviagen, untsville, AL 35805
2 Corresponding author: ewong{at}vt.edu
The objective of this study was to investigate intestinal nutrient transporter and enzyme mRNA in broilers selected on corn- and soybean-based (line A) or wheat-based (line B) diets. We investigated the peptide transporter PepT1, 10 amino acid transporters (rBAT, bo,+AT, ATBo,+, CAT1, CAT2, LAT1, y+LAT1, y+LAT2, BoAT, and EAAT3), 4 sugar transporters (SGLT1, SGLT5, GLUT5, and GLUT2), and a digestive enzyme (aminopeptidase N). Intestine was collected at embryo d 18 and 20, day of hatch, and d 1, 3, 7, and 14 posthatch. The mRNA abundance of each gene was assayed using real-time PCR and the absolute quantification method. For PepT1, line B had greater quantities of mRNA compared with line A (P = 0.001), suggesting a greater capacity for absorption of amino acids as peptides. Levels of PepT1 mRNA were greatest in the duodenum (P < 0.05), whereas the abundances of SGLT1, GLUT5, and GLUT2 mRNA were greatest in the jejunum (P < 0.05). Abundances of EAAT3, bo,+AT, rBAT, BoAT, LAT1, CAT2, SGLT5, and aminopeptidase N mRNA were greatest in the ileum (P < 0.05). Quantities of PepT1, EAAT3, BoAT, SGLT1, GLUT5, and GLUT2 mRNA increased linearly (P < 0.01), whereas CAT1, CAT2, y+LAT1, and LAT1 mRNA decreased linearly (P < 0.05) with age. Abundance of y+LAT2 mRNA changed cubically (P = 0.002) with peaks of expression at day of hatch and d 7, and aminopeptidase N and SGLT5 mRNA changed quadratically (P = 0.005) with age. These results provide a comprehensive profile of the temporal and spatial expression of nutrient transporter mRNA in the small intestine of chicks.
Key Words: amino acid transporter • broiler • monosaccharide transporter • PepT1
1 This project was supported by National Research Initiative Competitive Grant no. 2005-35206-15271 from the USDA Cooperative State Research, Education, and Extension Service. This research was also funded in part by the John Lee Pratt Animal Nutrition Program at Virginia Tech.
This article has been cited by other articles:
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  C. R. Mott, P. B. Siegel, K. E. Webb Jr., and E. A. Wong Gene Expression of Nutrient Transporters in the Small Intestine of Chickens from Lines Divergently Selected for High or Low Juvenile Body Weight Poult. Sci., November 1, 2008; 87(11): 2215 - 2224. [Abstract] [Full Text] [PDF]
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 E. R. Gilbert, E. A. Wong, and K. E. Webb Jr. BOARD-INVITED REVIEW: Peptide absorption and utilization: Implications for animal nutrition and health J Anim Sci, September 1, 2008; 86(9): 2135 - 2155. [Abstract] [Full Text] [PDF]
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