HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Andreatti Filho, R. L. Articles by Hargis, B. M. Search for Related Content PubMed PubMed Citation Articles by Andreatti Filho, R. L. Articles by Hargis, B. M.

Ability of Bacteriophages Isolated from Different Sources to Reduce Salmonella enterica Serovar Enteritidis In Vitro and In Vivo

R. L. Andreatti Filho^*, J. P. Higgins, S. E. Higgins, G. Gaona, A. D. Wolfenden, G. Tellez and B. M. Hargis^,1

^* College of Veterinary Medicine and Animal Science, Sao Paulo State University, Botucatu, Sao Paulo, Brazil, 18618-000; and Department of Poultry Science, University of Arkansas, Fayetteville 72701

¹ Corresponding author: bhargis{at}uark.edu

Salmonella enterica serovar Enteritidis-lysing bacteriophages isolated from poultry or human sewage sources were used to reduce Salmonella Enteritidis in vitro and in experimentally infected chicks. Cocktails of 4 different bacteriophages obtained from commercial broiler houses (CB4Ø) and 45 bacteriophages from a municipal wastewater treatment plant (WT45Ø) were evaluated. In experiment 1, an in vitro crop assay was conducted with selected bacteriophage concentrations (10⁵ to 10⁹ pfu/mL) to determine ability to reduce Salmonella Enteritidis in the simulated crop environment. Following 2 h at 37°C, CB4Ø or WT45Ø reduced Salmonella Enteritidis recovery by 1.5 or 5 log, respectively, as compared with control. However, CB4Ø did not affect total SE recovery after 6 h, whereas WT45Ø resulted in up to a 6-log reduction of Salmonella Enteritidis. In experiment 2, day-of-hatch chicks were challenged orally with 3 x 10³ cfu/chick Salmonella Enteritidis and treated cloacally with 1 x 10⁹ WT45Ø pfu/chick 1 h postchallenge. One hour later, chicks were treated or not with a commercially available probiotic (Floramax-B11). Both treatments significantly reduced Salmonella Enteritidis recovery from cecal tonsils at 24 h following vent lip application as compared with controls, but no additive effect was observed with the combination of bacteriophages and probiotic. In experiment 3, day-of-hatch chicks were challenged orally with 9 x 10³ cfu/chick Salmonella Enteritidis and treated via oral gavage with 1 x 10⁸ CB4Ø pfu/chick, 1.2 x 10⁸ WT45Ø pfu/chick, or a combination of both, 1 h postchallenge. All treatments significantly reduced Salmonella Enteritidis recovered from cecal tonsils at 24 h as compared with untreated controls, but no significant differences were observed at 48 h following treatment. These data suggest that some bacteriophages can be efficacious in reducing SE colonization in poultry during a short period, but with the bacteriophages and methods presently tested, persistent reductions were not observed.

Key Words: Salmonella enterica serovar Enteritidis • bacteriophage • crop assay • probiotic • chicken

This article has been cited by other articles:

				J. C. Noujaim, R. L. A. Filho, E. T. Lima, A. S. Okamoto, R. L. Amorim, and R. T. Neto Detection of T Lymphocytes in Intestine of Broiler Chicks Treated with Lactobacillus spp. and Challenged with Salmonella enterica Serovar Enteritidis Poult. Sci., May 1, 2008; 87(5): 927 - 933. [Abstract] [Full Text] [PDF]