HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Constantinoiu, C. C. Articles by Coleman, G. T. Search for Related Content PubMed PubMed Citation Articles by Constantinoiu, C. C. Articles by Coleman, G. T.

Purification of Immunoglobulins from Chicken Sera by Thiophilic Gel Chromatography

C. C. Constantinoiu^*,1, J. B. Molloy, W. K. Jorgensen and G. T. Coleman^*

^* School of Veterinary Science, University of Queensland, Brisbane, Queensland, 4072, Australia; and Department of Primary Industries and Fisheries, Yeerongpilly, Australia

¹ Corresponding author: c.constantinoiu{at}uq.edu.au

Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum. Data are provided on the recovery and purity of IgY obtained using potassium sulfate buffers of different concentrations. Decreasing the strength of potassium sulfate buffer from 0.5 to 0.3 M did not alter the amount of IgY recovered but increased the purity. Using 0.3 M potassium sulphate, we recovered approximately 63.7% of the serum Ig as almost pure IgY.

Key Words: immunoglobulin Y • serum • purification • thiophilic chromatography