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Arginine and Vitamin E Modulate the Subpopulations of T Lymphocytes in Broiler Chickens

S. T. Abdukalykova^*, X. Zhao^* and C. A. Ruiz-Feria^,1

^* Department of Animal Science, McGill University, Ste. Anne de Bellevue, H9X 3V9, Québec, Canada; and Department of Poultry Science, Texas A&M University, College Station 77843

¹ Corresponding author: ciro.ruiz{at}poultry.tamu.edu

We examined the effects of vitamin E (VE) and Arg on the subpopulations of T lymphocytes in peripheral blood after an infectious bursal disease virus vaccination (20 d old, n = 54). Broiler chickens were fed diets with normal levels of Arg (NARG, 1.2%) or high levels of Arg (HARG, 2.2%) and 3 levels of VE (40, 80, or 200 IU/kg of feed) in a factorial arrangement of treatments. The percentages of CD4+ and CD8+ cells 9 d after vaccination were not different in birds fed the HARG or NARG feed, but they were higher in birds fed the VE80 diet than in birds fed the VE40 diet. Birds fed the VE200 feed had similar levels of CD4+ and CD8+ cells as birds fed the VE40 diet. However, 19 d after vaccination, the percentage of CD4+ cells was higher in birds fed the HARG (43.93 ± 1.05) diet than in birds fed the NARG diet (41.14 ± 1.05) and in birds fed the VE80 and VE200 (44.1 ± 1.28 and 44.42 ± 1.28, respectively) diets compared with birds fed the VE40 diet (39.04 ± 1.28). On the other hand, the percentage of CD8+ cells was highest in birds fed the HARG-VE80 feed compared with all the other Arg and VE combinations. The percentages of CD3+ cells and BU-1+ cells were higher in birds fed the HARG (81.35 ± 1.46 and 22.3 ± 0.89, respectively) diet than in birds fed the NARG diets (77.82 ± 1.46 and 18.93 ± 0.89, respectively). Birds fed the VE40 had a higher amount of BU-1+ (24.35 ± 1.073) but lower amounts of CD3+ cells (75.76 ± 1.76) than birds fed the VE80 (18.46 ± 1.073 and 81.67 ± 1.76) and VE200 (19.03 ± 1.073 and 81.19 ± 1.76, respectively). Neither Arg nor VE had an effect on the CD4+:CD8+ cell ratio and on the percentage of immature (CD4+CD8+) T lymphocytes 9 d after vaccination. These results suggest that Arg and VE have complimentary effects on cellular and humoral immune function and may enhance the resistance of broilers to infectious diseases.

Key Words: arginine • vitamin E • T lymphocyte • infectious bursal disease virus