HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wang, Z. Y. Articles by Zhou, Q. Y. Search for Related Content PubMed PubMed Citation Articles by Wang, Z. Y. Articles by Zhou, Q. Y.

A Comparison of Methods to Determine Amino Acid Availability of Feedstuffs in Cecectomized Ganders¹

College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, P. R. China

² Corresponding author: dkwzy{at}263.net or ssr236{at}163.com

Experiments were conducted to compare endogenous amino acid losses and the true amino acid availability (TAAA) of 3 feedstuffs by using methods involving a short-term fasting and an N-free diet with cecectomized ganders. Diets were formulated to contain soybean meal, fish meal, and cottonseed meal as the sole source of protein. A precision-fed assay was used in which each feed sample was precise-fed (60 g) to geese and excreta were collected for 48 h. A N-free diet and fasting methods were used to evaluate the endogenous amino acid losses. Endogenous losses of 3 amino acids were significantly different (P < 0.01) with the N-free diet and fasting methods. The TAAA of soybean meal, fish meal, and cottonseed meal determined by N-free diet method ranged from 84.49 to 97.09%, 89.18 to 98.16%, and 77.09 to 98.32%, respectively. The TAAA of these 3 diets determined by the fasting method ranged from 83.50 to 97.77%, 88.08 to 99.60%, and 76.09 to 98.09%, respectively. However, there were only a few small differences (P > 0.05) between methods in each amino acid. In conclusion, there was no difference in determination of the amino acid availability of these feedstuffs using cecectomized ganders between the N-free diet and fasting methods.

Key Words: amino acid availability • endogenous amino acid loss • nitrogen-free diet • cecectomized gander

¹ Supported by the Key Scientific and Technological Project of Jiangsu Province in the Tenth Five-Year, P. R. China (Grant No. BE2001369).