Poult. Sci.
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Poult Sci 2008. 87:226-232. doi:10.3382/ps.2007-00329
© 2008 Poultry Science Association
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GENETICS

Peroxisome Proliferator-Activated Receptor-{gamma} Gene: A Key Regulator of Adipocyte Differentiation in Chickens1

Y. Wang2, Y. Mu2, H. Li3, N. Ding, Q. Wang, Y. Wang, S. Wang and N. Wang

College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, P. R. China

3 Corresponding author: lihui{at}neau.edu.cnorlihui645{at}hotmail.com

The peroxisome proliferator-activated receptors (PPAR) are members of the nuclear hormone receptor superfamily. Peroxisome proliferator-activated receptor-{gamma} is regarded as a "master regulator" of adipocyte differentiation in mammals. The current study was designed to investigate the function and regulatory mechanism of PPAR{gamma} in chicken adipogenesis by RNA interference. Preadipocytes were isolated from the abdominal fat tissue of 12-d-old chickens and cultured. Small-interference PPAR{gamma} RNA (siPPAR{gamma}) was synthesized by in vitro transcription and transfected into chicken preadipocytes by using liposomes. The suppressive effect of siPPAR{gamma} was detected by real-time reverse-transcription PCR and reverse-transcription PCR. The results showed that transient transfection with siPPAR{gamma} significantly inhibited differentiation and enhanced proliferation of chicken preadipocytes (P < 0.05). The adipogenesis-associated adipocyte fatty acid-binding protein gene was down-regulated when PPAR{gamma} was silenced. The current work indicates that PPAR{gamma} is a key regulator of chicken preadipocyte differentiation.

Key Words: chicken preadipocyte • proliferation • differentiation • peroxisome proliferator-activated receptor-{gamma} • RNA interference

1 This research was supported by the National Basic Research Program of China (no. 2006CB102105), the National Natural Science Foundation Key Project of China (no. 30430510), and the Natural Science Foundation Key Project of Hei Longjiang Province, Harbin, China (no. ZJN0604-01).

2 These authors contributed equally to this work.




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