HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Fasina, Y. O. Articles by McKee, S. R. PubMed PubMed Citation Articles by Fasina, Y. O. Articles by McKee, S. R.

Intestinal Cytokine Response of Commercial Source Broiler Chicks to Salmonella Typhimurium Infection

Y. O. Fasina^*,1, P. S. Holt, E. T. Moran^*, R. W. Moore, D. E. Conner^* and S. R. McKee^*

^* Department of Poultry Science, Auburn University, 260 Lem Morrison Drive, Auburn, AL 36849-5416; and USDA-ARS, Egg Safety & Quality Research Unit, Russell Research Center, 950 College Station Road, Athens, GA 30605

¹ Corresponding author: fasinyo{at}auburn.edu

Development of molecular-based immunotherapeutic strategies for controlling Salmonella Typhimurium (ST) infection in poultry requires a better understanding of intestinal and cecal cytokine responses. Accordingly, an experiment was conducted to measure changes in intestinal cytokine expression when commercial source broiler chickens were challenged with a nalidixic acid-resistant ST. Ross broiler chicks were nonchallenged with ST (control treatment) or challenged by orally giving 7.8 x 10⁶ cfu at 4 d of age (STC treatment). Each treatment consisted of 4 replicate pens with 14 chicks per pen. Expression levels of proinflammatory cytokines, interferon-, and antiinflammatory interleukin (IL)-10 were determined at 5 and 10 d postchallenge (PC). Intestinal flushes were also collected from each treatment at 7 d PC to estimate IgA and IgG. Results showed an upregulation in IL-1β mRNA in STC chicks at 5 d PC. By 10 d PC, the expression of IL-1β was further increased and accompanied by an upregulation of IL-6 and interferon- mRNA, whereas IL-10 mRNA expression decreased. It was concluded that ST induced an intestinal mucosal inflammatory response in commercial source broiler chicks less than 2 wk of age.

Key Words: cytokine • Salmonella Typhimurium • intestine • commercial-type broiler chick