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The Effect of Fibroblast Growth Factor 2 on the In Vitro Expression of Syndecan-4 and Glypican-1 in Turkey Satellite Cells¹

S. G. Velleman^*,2, X. Li^*, C. S. Coy^* and D. C. McFarland

^* Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster 44691; and Department of Animal and Range Sciences, South Dakota State University, Brookings 57007

² Corresponding author: Velleman.1{at}osu.edu

The membrane-associated heparan sulfate proteoglycan families, consisting of the syndecans and glypicans, are low-affinity receptors for fibroblast growth factor 2 (FGF2) that are essential in regulating the cellular response to FGF2. Fibroblast growth factor 2 is a potent stimulator of skeletal muscle cell proliferation and a strong inhibitor of differentiation. The regulation of the expression of the syndecans and glypicans will likely play a role in modulating the effects of FGF2 on cellular growth properties. In the present study, the effect of FGF2 on the expression of syndecan-4 and glypican-1 was measured by real-time PCR during turkey myogenic satellite cell proliferation and differentiation in vitro. Both syndecan-4 and glypican-1 transcription were influenced by the addition of exogenous FGF2. Syndecan-4 mRNA expression was reduced only during proliferation, whereas glypican-1 expression was reduced during both proliferation and differentiation. These results suggest that FGF2 growth factor signaling is, in part, regulated by an autoregulatory loop involving FGF2 regulation of syndecan-4 and glypican-1 expression and will affect the growth of skeletal muscle by modulating the proliferation and differentiation of satellite cells.

Key Words: fibroblast growth factor 2 • glypican-1 • muscle • satellite cell • syndecan-4

¹ Salary and research support was provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, and to S. G. Velleman from the Cooperative State Research, Education, and Extension Service (CSREES), USDA, under agreement number 2003-35206-13696. Research support to S. G. Velleman and D. C. McFarland was provided by the Midwest Poultry Research Consortium and represents collaborative research between S. G. Velleman and D. C. McFarland as part of USDA CSREES multistate project NC 1131.