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Ethylenediaminetetraacetate and lysozyme improves antimicrobial activities of ovotransferrin against Escherichia coli O157:H7

K. Y. Ko^*, A. F. Mendoncam, H. Ismail^* and D. U. Ahn^*,1

^* Department of Animal Science, and Department of Food Science and Human Nutrition, Iowa State University, Ames 50011

¹ Corresponding author: duahn{at}iastate.edu

The aim of this study was to evaluate the effect of EDTA, lysozyme, or the combination of EDTA and lysozyme on the antibacterial activity of ovotransferrin against Escherichia coli O157:H7. Ovotransferrin solutions (20 mg/mL) containing 100 mM NaHCO₃ (OS) with added EDTA (2.0 or 2.5 mg/mL), lysozyme (1.0, 1.5, or 2.0 mg/mL), or both were prepared. The antibacterial activities of OS, OSE (OS + EDTA), or OSL (OS + lysozyme) against E. coli O157:H7 in model systems were investigated by turbidity and viability tests. In addition, OSE, OSL, or OSEL (OS + EDTA + lysozyme) was applied to irradiated pork chops and commercial hams to determine whether the solutions had antibacterial activity on meat products. The effect of the initial cell population on the antibacterial activity of OSE, OSL, and OSEL was determined. Ethylenediaminetetraacetate at 2 mg/mL plus OS induced a reduction of approximately 3 to 4 log in viable E. coli O157:H7 cells in brain heart infusion broth media, and 1 mg/mL of lysozyme plus OS resulted in a reduction of approximately 0.5 to 1.0 log during a 36-h incubation at 35°C. However, neither OSE nor OSEL showed a significant antibacterial effect on pork chops and hams during storage at 10°C. The initial cell number in media did not affect the antibacterial activity of OSE or OSEL against E. coli O157:H7. This study demonstrates that combinations of ovotransferrin, NaHCO₃, and EDTA have the potential to control E. coli O157:H7.

Key Words: ovotransferrin • antibacterial activity • ethylenediaminetetraacetate • lysozyme • Escherichia coli O157:H7