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Distribution of vasoactive intestinal peptide and substance P messenger ribonucleic acid in intestinal nerve of Remak of chicken¹

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

³ Corresponding author: chenqsh305{at}yahoo.com.cn

The intestinal nerve of Remak (INR) is unique to birds. The exact distributions and secretions of the peptide transmitters in the INR that innervate the intestine, and the patterns of their projections to the nerve plexus of the intestinal wall, are still unclear. In the present study, the distributions of chicken vasoactive intestinal peptide (VIP) mRNA and substance P (SP) mRNA in the chicken INR were demonstrated by means of in situ hybridization histochemistry, using sense and antisense digoxigenin-labeled RNA probes. The in situ hybridization histochemistry results suggested that VIP mRNA-positive neurons were mainly distributed in ganglions, whereas some of the INR fibers were weakly positive. The proportion of VIP mRNA-positive cells among total neurons was 75% in the juxta-jejunoileum portion and 87% in the juxta-rectal portion of the INR. Most SP mRNA-positive cells were sporadically distributed in layers or small groups within the ganglions, as well as in nerve trunks between ganglions. Moreover, the proportion of positive cells among total neurons was 83% in the juxta-jejunoileum portion and 98% in the juxta-rectal portion of the INR, which suggests that both VIP and SP mRNA are coexpressed in the chicken INR. Our results provide an approach to further study of the locations of VIP and SP in nerve tissue.

Key Words: chicken intestinal nerve of Remak • vasoactive intestinal peptide messenger ribonucleic acid • substance P messenger ribonucleic acid • in situ hybridization • ribonucleic acid probe

¹ This study was supported by grants (no 30671513 and 30871833) from the National Science Foundation of China.

² These authors have contributed equally to this work.