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Prostaglandin F₂ receptor in the neurohypophysis of hens

Department of Avian Endocrinology, Gifu University, Yanagido, Gifu 501-1193, Japan

¹ Corresponding author: kawasima{at}gifu-u.ac.jp

To elucidate whether the receptor for prostaglandin (PG) F₂, one of PG, exists in the neurohypophysis in hens and whether the binding of receptor changes with relation to oviposition, the PGF₂ binding component in the membrane fraction of the neurohypophysis of laying hens was analyzed by radioligand binding assay using [5,6,8,9,11,12,14,15(n)-³H]PGF₂. The binding component had characteristics of a receptor such as binding specificity, high affinity, and limited capacity for PGF₂. Scatchard analysis indicated that the binding site was of a single class. The binding capacity of the receptor was smaller in laying hens than in nonlaying hens, whereas the binding affinity was not significantly different between these hens. When non-laying hens received an i.m. injection of estradiol-17β or progesterone (0.5 mg/hen), the specific binding of the PGF₂ receptor in the neurohypophysis was decreased. In laying hens, the specific binding decreased and the blood arginine vasotocin (AVT) concentration increased just after oviposition but did not change during a 24-h day in nonlaying hens. An i.v. injection of PGF₂ (2 µg/hen) induced oviposition and caused an increase in the blood AVT concentration with a decrease in the specific binding of PGF₂ receptor. The present study suggests a possibility that PGF₂ may directly cause the AVT release from the neurohypophysis at oviposition time in hens.

Key Words: hen neurohypophysis • prostaglandin F₂ receptor • receptor binding • arginine vasotocin release • oviposition

This article has been cited by other articles:

				T. Takahashi and M. Kawashima Properties of estrogen binding components in the plasma membrane of neurohypophysis in hens and changes in its binding before and after oviposition Poult. Sci., October 1, 2009; 88(10): 2206 - 2211. [Abstract] [Full Text] [PDF]