Poult. Sci.
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Poult Sci 2010. 89:88-95. doi:10.3382/ps.2009-00289
© 2010 Poultry Science Association
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MOLECULAR, CELLULAR, AND DEVELOPMENTAL BIOLOGY

Adipogenesis in ducks interfered by small interfering ribonucleic acids of peroxisome proliferator-activated receptor {gamma} gene

M. Xiong, S. Li, X. Peng, Y. Feng, G. Yu, Q. Xin and Y. Gong1

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, P. R. China

1 Corresponding author: xiongminzw{at}yahoo.com.cn

Peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}) participates in adipocyte differentiation and maintenance, including the promotion of lipid storage in mammals. In the present study, 3 duck PPAR{gamma} small interfering RNA (siRNA) expression plasmids were constructed to investigate the effect of downregulating the expression of PPAR{gamma} on adipogenesis and fat accumulation in ducks. The results indicate that the 3 siRNA specific for conserved regions of PPAR{gamma} can effectively inhibit expression of PPAR{gamma}. It was demonstrated that the expression of lipoprotein lipase and adipocyte fatty acid-binding protein in duck adipose tissue is repressed when the expression of PPAR{gamma} is downregulated by siRNA. At the same time, the weight of abdominal fat at 21 and 35 d of age is decreased significantly (P < 0.05) compared with the control. However, the triglyceride levels in serum and muscle are not affected when the mRNA of PPAR{gamma} is repressed. The current study indicates that the suppression of PPAR{gamma} reduces abdominal fat deposition and regulates adipogenesis in ducks.

Key Words: duck • peroxisome proliferator-activated receptor {gamma} • adipogenesis • small interfering ribonucleic acid






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