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PROCESSING, PRODUCTS, AND FOOD SAFETY |


* Department of Poultry Science, University of Arkansas, Fayetteville 72701; and
Department of Food Science, University of Arkansas, Fayetteville 72704
1 Corresponding author: cmowens{at}uark.edu
| ABSTRACT |
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Key Words: marination consumer broiler salt
| INTRODUCTION |
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With the increasing demand for poultry boneless breast meat, producers are shortening the deboning time, and meat quality properties are directly affected as a result. A positive correlation was reported by Stewart et al. (1984) between prerigor deboning and shear values. Stewart et al. concluded that breast meat toughness associated with prerigor deboning can be evaded by application of appropriate tenderizing treatment or postrigor (at least 4 to 6 h postmortem) deboning of breast muscle. Some of the technologies with promising results in improving tenderness of early harvested broiler breast meat include electrical stimulation (Sams et al., 1989), wing restraints or tensioning (Birkhold and Sams, 1993), and high temperature conditioning of broiler carcasses (Sams, 1990), but none of these technologies fully assure acceptable broiler breast tenderness and product uniformity or are widely used in industry.
The meat industry has successfully implemented marination technologies to meet consumer demands and help maintain product uniformity, and the use of marination has increased dramatically in the past few years in the poultry industry. It is one of the methods used by the industry for tenderization of early harvested broiler breast fillets. Zheng et al. (2000) reported that poultry breast fillets deboned at 2 h postmortem and marinated with salt and phosphate (sodium tripolyphosphate, hexametaphosphate, or tetrasodium pyrophosphate) significantly reduced the toughness of poultry breast. Additionally, beneficial effects of marination on meat texture include a juicier meat and reduction of water loss during cooking (Miller, 1998).
Most commonly used commercial marinades contain salt, phosphate, and water as their primary ingredients (Barbut et al., 1988; Lyon et al., 2005). Phosphates are known to increase water holding capacity (WHC), stabilize meat emulsions, improve juiciness and tenderness, and maintain flavor of processed meat products (Ellinger, 1972). Phosphates, like sodium tripolyphosphate, improve the functional properties of meat proteins by increasing the ionic strength and pH of meat (Hamm, 1960). Increased ionic strength or greater pH results in greater WHC or juicier product. Salt or sodium chloride solubilize proteins to increase WHC (Whiting, 1988). The Cl– ions interact with positively charged myosin molecules and result in a change of isoelectric point to a lower pH (Hamm, 1986). This weakens the interaction and increases swelling and WHC (Hamm, 1986). Increasing the WHC of the meat reduces cook loss and increases tenderness and juiciness of the meat product (Desmond, 2006).
There are concerns of increased sodium intake in consumer diets due to the association of excessive NaCl or sodium intake and its relationship to hypertension (Dahl, 1972; Fries, 1976; Law et al., 1991a,b). These concerns have prompted processors to decrease salt in many meat products. Reduced salt levels in meat lower the ionic strength and may not allow an increase in the WHC compared with meat with greater salt concentrations. Salt is a natural flavor enhancer, increasing the flavor intensity of meat products (Gillette, 1985). Thus, salt reduction not only reduces the perceived saltiness but also weakens the overall flavor in meat products (Wu and Smith, 1987; Ruusunen et al., 2005). Sodium content in meat products can be lowered by NaCl reduction, substitution, or both with other ingredients like potassium chloride or magnesium chloride (Terrell and Olson, 1981; Terrell, 1983).
The purpose of this study was to produce a marinated product with reduced NaCl without negatively affecting the sensory attributes. Therefore, the objective of this study was to evaluate and compare the quality characteristics of different sodium levels by varying NaCl content in marinated poultry breast meat.
| MATERIALS AND METHODS |
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At 42 d of age, 100 commercial broilers were processed in 2 replications at the university poultry processing pilot plant. Feed was withdrawn 10 h before processing; however, broilers had free access to water before processing. Broilers were transported in coops to the processing plant 15 min before slaughter. The broilers were electrically stunned (11 mV, 10 s), manually cut (severed left carotid artery and jugular vein), bled out (1.5 min), scalded (55°C, 2 min), and picked inline using commercial defeathering equipment. Carcasses were manually eviscerated, and a 2-stage immersion or water chilling system was used for all treatments. Carcasses were placed in a prechill tank for 15 min (12°C) and a chill tank (ice slush) for 55 min (1°C); carcasses were manually agitated frequently throughout the chilling process to reduce thermal layering around the carcass and improve chilling efficiency. The carcasses were deboned at 5 h postmortem. In efforts to primarily determine the appropriate flavor and saltiness of a product, postrigor deboned fillets were evaluated in this study.
Marination
Following chilling, breast fillets were aged on ice for 24 h. Following aging, fillets were marinated in 2 replications (20 fillets per replication per treatment) in a vacuum tumbler (508 mm Hg; 67.5 kPa) at 20 rpm for 30 min in a cooler at 4°C in a 15% marinade solution (based on meat weight) consisting of varying levels of NaCl (salt) and 0.45% sodium tripolyphosphate. The salt concentrations used for this experiment were 0.0, 0.5, 0.75, 1.0, and 1.25%. The control (0%) was not marinated with salt or phosphate. After tumbling, fillets were allowed to rest for a period of 5 min and then vacuum packed and frozen. The left breast fillet was used for consumer sensory evaluation and the right fillet for instrumental Meullenet-Owens Razor Shear analysis (MORS).
Cooking
Before cooking, the frozen fillets were taken out of the freezer (–20°C) and thawed overnight in a refrigerator (4°C). The fillets were cooked in pans covered with aluminum foil to an internal temperature of 76°C in a convection oven according to the method described by Sams (1990). Cooked fillets were used for sensory or instrumental analysis.
Consumer Sensory Analysis
The 5 treatments were evaluated by a 68-member consumer panel. The consumer test was conducted at the University of Arkansas Sensory Laboratory. The sensory testing facility consisted of 6 individual testing booths with controlled lighting and positive airflow. The panelists were presented with one treatment at a time that consisted of three 1- to 1.25-cm cubes of breast meat identified by a random 3-digit code (Meilgaard et al., 1999). Room-temperature water and unsalted crackers were also served. A total of 5 treatments were served, and treatment order was completely randomized for each panelist. Once the consumer received the sample, they were asked to enter the code into the computer and evaluate the product for overall impression, flavor, and texture on a 9-point hedonic scale with 1 = dislike extremely and 9 = like extremely. The 5-point just about right (JAR) scale was used on questions about tenderness, moistness, overall flavor, chicken flavor, and saltiness. In this case, 1 = much too low and 5 = much too high with 3 = JAR.
Instrumental Shear Analysis
After cooking, fillets were cooled, wrapped in aluminum foil, and stored overnight at 4°C. On the following day, fillets were evaluated for tenderness using the MORS method (Cavitt et al., 2004), which consists of shearing the sample perpendicularly to the longitudinal fiber orientation with a razor blade in duplicate at predetermined locations. The MORS total energy (MORSE, N·mm) was determined using a Texture Analyzer (model TX-TX2, Texture Technologies, Scarsdale, NY) with a 5-kg load cell using a razor blade probe with a height of 24 mm and a width of 8.9 mm set to a penetration depth of 20 mm. The crosshead speed was 5 mm/s; sample shear depth was 20 mm; and trigger force was 0.1 N. Data points were collected with an acquisition rate of 200 points/s. The instrumental data were collected using Texture Exponent 32 version 1.0.0.92. The MORSE was calculated from the force-time curves using the macro options of Texture Exponent (Stable Micro Systems, Godalming, Surrey, UK).
Statistical Analysis
All data was subjected to ANOVA conducted using JMP (version 5.1, SAS Institute, 2004). Means from sensory analysis were separated using least squares means at a significance level of P < 0.05. Salt concentration (0.0, 0.5, 0.75, 1.0, and 1.25%) was defined as the main effect to determine the influence of salt concentration on meat quality attributes. The frequency distributions for the hedonic scales and JAR scales were compared using Fishers exact test for equality of distributions. A chi-square test was not utilized in this instance because more than 20% of the expected values were below 5 (Fleiss, 2003). Significant differences for the frequency distribution were determined at a P < 0.05.
| RESULTS AND DISCUSSION |
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4) the nonmarinated control treatment. Furthermore, these percentages in the dislike categories (hedonic scale rating
4) were significantly greater than the percentages in the same categories for the marinated fillets regardless of salt (0.5 to 1.25%) where only 5.88 to 8.82% of the consumers disliked (hedonic scale rating
4) the marinated fillets. In comparison, 83.81 to 88.23% of the consumers liked (hedonic scale rating
6) the marinated fillets regardless of their salt concentration. Furthermore, greater than 80% of consumers liked (hedonic scale rating
6) the texture of marinated fillets compared with approximately 40% of consumers who like the texture of nonmarinated fillets. Saha et al. (2006) reported that mean consumer ratings for marinated fillets ranged from like slightly to like moderately regardless of debone time (pre- or postrig-or), suggesting that marination results in acceptable texture. The myofibrillar proteins of muscle, myosin and actin, are salt soluble, and these proteins impact a wide range of meat attributes including texture and WHC (Foegeding, 1987).
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6) all of the marinated fillets, whereas only 38% of consumers liked the nonmarinated fillets in terms of flavor. There was generally a greater percentage of consumers who disliked the flavor of the non-marinated fillets compared with the marinated fillets. There were no differences in percentages of consumers who disliked (hedonic scale rating
4) flavor among marinated fillets (0.5% salt or greater). Bryhni et al. (2003) reported that flavor characteristics negatively influenced consumer liking. Howe and Barbella (1937) commented that the addition of salt to meat develops the flavor. Since then, numerous researchers have found that marination with salt and phosphate improves meat flavor (Cross and Stanfield, 1976; Schwartz and Mandigo, 1976; Olson, 1982; Steinhauer, 1983; Wheeler et al., 1990; Miller, 1998; Woods and Church, 1999).
In addition to hedonic sensory scales, JAR sensory scales were used to determine appropriateness of meat tenderness (Table 3
). Fillets marinated with any concentration of salt (0.5, 0.75, 1.0, 1.25%) had greater (P < 0.05) tenderness JAR mean values compared with the nonmarinated control treatment. Furthermore, JAR tenderness mean values for all the marinated fillets were 2.99 to 3.03, indicating the consumers considered the fillets to be JAR, whereas the nonmarinated fillets had a mean of 2.4, which indicated that consumer considered these as too tough. Furthermore, there was a greater percentage of consumers who considered the nonmarinated fillets as much too tough or too tough compared with marinated fillets (Table 4
). The percentage of consumers who considered the fillets as JAR for tenderness was significantly greater in all marinated fillets (>77%) compared with nonmarinated fillets (32%). Moreover, the percentages of consumers considering the meat as JAR in any JAR scale category were similar (P > 0.05) for all marinated fillets. These data suggest that marination can improve consumer perception of poultry meat tenderness. This research also agrees with previous research that has shown that marination improves meat quality attributes (Lyon et al., 1998). Saha (2007) reported that the percentage of consumers rating meat tenderness as JAR ranged from 59.3 to 81.1% for marinated fillets in comparison with 21.6 to 82.4% (Xiong et al., 2006) and 25.0 to 73.0% (Cavitt et al., 2005a) for nonmarinated pre- and postrigor fillets. Palladino and Ball (1979) found sodium ions have a tenderizing effect in postrigor broiler breast meat. Robbins et al. (2003) reported that marinated beef steaks and roasts were considered to be significantly more tender and juicy than nonmarinated controls by consumer sensory panel. Similar results were also reported by Robbins et al. (2002) for evaluation of marinated beef using trained sensory panel. Sheard et al. (1999) and Smith et al. (1984) reported that phosphate-injected pork roasts were more tender than fresh cooked, unpumped roasts.
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Overall flavor JAR mean values for breast fillets increased with the increasing salt concentration (Table 3
). Fillets marinated with 1.25% salt had the highest JAR mean, indicating JAR, and the nonmarinated fillets had the lowest means, indicating too weak, whereas the other marinated fillets (0.5, 0.75, and 1.0%) were intermediate. These results are in accordance with hedonic scale distributions where majority of the consumers liked (hedonic scale rating
6) the marinated fillets compared with the nonmarinated treatment. Fillets marinated with salt concentrations of 1.0 and 1.25% had significantly (P < 0.05) lower percentages (16.18 and 14.71%, respectively) of consumers considering them as too weak in comparison with the nonmarinated control fillets (38.24%; Table 4
). Fillets marinated with 0.5 or 0.75% had similar percentage as those with greater (1.0 or 1.25%) or lower (0%) salt concentration. Similar results were reported by Keeton (1983) and Hayes et al. (2006) for pork loins. The authors found that pork loins enhanced with phosphate and salt had a greater overall flavor rating in comparison with the control. In this study, most consumers considered marinated fillets regardless of salt concentration to be JAR in overall flavor.
Consumers considered the chicken flavor to be uniform with marination or salt concentration having no effect on the JAR means (Table 3
). Terrell (1983), Ruusunen and Puolanne (2005), and Desmond (2006) commented on salt being essential for the flavor and it being used by the meat industry as flavoring. Therefore, salt may mask some of the chicken flavor, resulting in a lower percentage of consumers considering the marinated treatments as too strong and much too strong.
All JAR saltiness mean scores ranged from 2.60 to 3.20 for marinated treatments (Table 3
). The nonmarinated control fillets had a significantly lower JAR rating (1.89) in comparison with the marinated samples, indicating that nonmarinated samples were not salty enough as would be expected. Salt concentration of 1.25% had the greatest JAR rating of 3.2, indicating that more consumers rated the samples as too salty rather than JAR. Among the marinated samples, fillets with 0.5% salt concentration had lowest rating, followed by the 0.75 and 1.0 salt concentrations suggesting that as salt concentration increases, the appropriateness of saltiness improves in this range. Frequency distribution (%) for saltiness had differences in the JAR ratings (Table 4
). As the percentage of salt in the formulation increased, the percentage of consumers who considered the product as not salty enough generally decreased. Similarly, the saltiness was considered as not salty enough by 14.71 to 36.76% of consumers for the marinated treatments. Consumers considering the nonmarinated fillets as not nearly salty enough or not salty enough were significantly greater (25.0 and 58.82%, respectively) in comparison with the marinated samples (<4.41 and <36.76%, respectively). The sensory attributes including saltiness had the greatest JAR proportions for the 0.75% salt concentration, indicating this concentration as being accurate for ready-to-cook products. Raw marinated breast fillets fall under the category of further processed products that generally undergo further preparation after purchase (e.g, retail raw marinated breast fillets). For example, in household settings, it is likely that chicken breasts would be prepared with additional ingredients (seasonings, spices, etc.) leading to increased levels of appropriateness for flavor, saltiness, or both. Therefore, using a greater salt concentration for marination may potentially result in a product being categorized by a large percentage of people as too salty after cooking. Salt concentration of 0.5% had a significantly (P < 0.05) greater percentage of consumers rating it as not salty enough in comparison with other marinated treatments and significantly (P < 0.05) lower percentage in comparison with the nonmarinated control. Also, the fillets were not penalized for being too salty unlike salt concentrations of 1.0 and 1.25%. Over 50% of consumers considered saltiness levels of the 0.5% marinated fillets as JAR. However, when marinating with 0.75%, the majority of consumers (>70%) considered the saltiness levels as JAR. Fillets with salt concentrations of 1% and above were considered as too strong for overall flavor, and >20% of the consumers considered them to be too salty. These results indicate that the lower salt concentrations used in marinating breast fillets would be appropriate to produce products that are acceptable to consumers.
Conclusions
Marination improved consumer acceptance of broiler breast fillets for all quality attributes including overall impression, flavor, texture, tenderness, moistness, and saltiness, regardless of salt concentration. Saltiness, as perceived by consumers, increased as salt concentrations in the marinades increased. Salt concentrations of 1% or greater resulted in greater than 20% of consumers who considered the fillets as too salty, which could result in more consumer complaints in the marketplace. The majority of consumers considered the lower levels of salt to be just about right for saltiness levels, whereas very few consumers considered the fillets as too salty. The results of this study suggest that using low salt concentrations, 0.5% to 0.75% (depending on end product), is appropriate for marination of postrigor broiler breast meat to obtain desirable quality attributes. Future research should include evaluation of tenderness of early deboned fillets marinated with low salt concentrations.
Received for publication June 6, 2008. Accepted for publication October 24, 2008.
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