Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits

Unitat de Ciencia Animal, Departament de Ciencia Animal i dels Aliments, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Bellaterra 08193, Spain. Marcel.Amills@uab.es

The chicken insulin-like growth factor (IGF)1 and IGF2 genes have been partially sequenced in six individuals of each of two chicken strains of the Black Penedesenca breed (PN and MN). These two strains are genetically diverse for growth traits. Sequence alignment revealed the existence of three single nucleotide polymorphisms (SNP) (IGF1-SNP1, IGF2-SNP2, and IGF2-SNP3). These three SNP and a fourth IGF1 polymorphism (IGF1-SNP4) were typed in 60 individuals from each strain by using PCR-RFLP or primer extension analysis. No significant associations among these four SNP, growth traits, and plasma IGF1 concentration were identified. In contrast, suggestive associations (P < or = 0.05) were found between IGF1-SNP1 and average daily gain at 107 d and feed efficiency at 44, 73, and 107 d. However, these associations were not simultaneously found in both strains suggesting that they might have been produced by linkage disequilibrium with another mutation located in the IGF1 locus or another linked gene. Since the PN and MN strains differ very markedly on their feed intake, the chicken leptin gene was included in the sequence analysis. Unfortunately, attempts to amplify several regions of this gene were unsuccessful. Even when primers complementary to highly conserved regions were used, the PCR consistently failed. Other authors have reported similar problems when trying to amplify avian leptin sequences.

This article has been cited by other articles:

				C. G. Scanes Absolute and Relative Standards--The Case of Leptin in Poultry: First Do No Harm Poult. Sci., October 1, 2008; 87(10): 1927 - 1928. [Full Text] [PDF]

				G. Hen, S. Yosefi, A. Ronin, P. Einat, C. I Rosenblum, R. J Denver, and M. Friedman-Einat Monitoring leptin activity using the chicken leptin receptor J. Endocrinol., May 1, 2008; 197(2): 325 - 333. [Abstract] [Full Text] [PDF]

				H. Adachi, Y. Takemoto, T. Bungo, and T. Ohkubo Chicken leptin receptor is functional in activating JAK-STATpathway in vitro J. Endocrinol., May 1, 2008; 197(2): 335 - 342. [Abstract] [Full Text] [PDF]

				A. Zidi, A. Sanchez, G. Obexer-Ruff, and M. Amills Sequence Analysis of Goat Major Histocompatibility Complex Class I Genes J Dairy Sci, February 1, 2008; 91(2): 814 - 817. [Abstract] [Full Text] [PDF]

				J. Estany, M. Tor, D. Villalba, L. Bosch, D. Gallardo, N. Jimenez, L. Altet, J. L. Noguera, J. Reixach, M. Amills, et al. Association of CA repeat polymorphism at intron 1 of insulin-like growth factor (IGF-I) gene with circulating IGF-I concentration, growth, and fatness in swine Physiol Genomics, October 19, 2007; 31(2): 236 - 243. [Abstract] [Full Text] [PDF]

				M. P. Richards and M. Proszkowiec-Weglarz Mechanisms Regulating Feed Intake, Energy Expenditure, and Body Weight in Poultry Poult. Sci., July 1, 2007; 86(7): 1478 - 1490. [Abstract] [Full Text] [PDF]

				B. Badaoui, J. M. Serradilla, A. Tomas, B. Urrutia, J. L. Ares, J. Carrizosa, A. Sanchez, J. Jordana, and M. Amills Short Communication: Identification of Two Polymorphisms in the Goat Lipoprotein Lipase Gene and Their Association with Milk Production Traits J Dairy Sci, June 1, 2007; 90(6): 3012 - 3017. [Abstract] [Full Text] [PDF]

				M. Lei, C. Luo, X. Peng, M. Fang, Q. Nie, D. Zhang, G. Yang, and X. Zhang Polymorphism of Growth-Correlated Genes Associated with Fatness and Muscle Fiber Traits in Chickens Poult. Sci., May 1, 2007; 86(5): 835 - 842. [Abstract] [Full Text] [PDF]

				B. Badaoui, J. M. Serradilla, A. Tomas, B. Urrutia, J. L. Ares, J. Carrizosa, A. Sanchez, J. Jordana, and M. Amills Goat Acetyl-Coenzyme A Carboxylase {alpha}: Molecular Characterization, Polymorphism, and Association with Milk Traits J Dairy Sci, February 1, 2007; 90(2): 1039 - 1043. [Abstract] [Full Text] [PDF]

				H. Zhou, N. Deeb, C. M. Evock-Clover, C. M. Ashwell, and S. J. Lamont Genome-wide linkage analysis to identify chromosomal regions affecting phenotypic traits in the chicken. I. Growth and average daily gain. Poult. Sci., October 1, 2006; 85(10): 1700 - 1711. [Abstract] [Full Text] [PDF]

				Q. Nie, B. Sun, D. Zhang, C. Luo, N. A. Ishag, M. Lei, G. Yang, and X. Zhang High Diversity of the Chicken Growth Hormone Gene and Effects on Growth and Carcass Traits J. Hered., November 1, 2005; 96(6): 698 - 703. [Abstract] [Full Text] [PDF]

				M. Amills, O. Vidal, L. Varona, A. Tomas, M. Gil, A. Sanchez, and J. L. Noguera Polymorphism of the pig 2,4-dienoyl CoA reductase 1 gene (DECR1) and its association with carcass and meat quality traits J Anim Sci, March 1, 2005; 83(3): 493 - 498. [Abstract] [Full Text] [PDF]